Description
Technical Monograph: T-B5-00 | Actin-Sequestering Research Reagent
Product Code: HRL-TB5-10 / HRL-TB5-20 | Total Peptide Mass: 10 mg / 20 mg
This high-purity synthetic peptide is the active (17-23) fragment of the endogenous protein Thymosin Beta-4. Engineered for advanced cellular biology research, this variant is optimized for investigating the regulation of the actin cytoskeleton and the modulation of cellular migration and differentiation in vitro.
Molecular Specification & Composition
| Attribute | Technical Detail |
| Sequence Fragment | Ac-Ser-Asp-Lys-Pro-Asp-Met-Ala-Glu-Ile-Glu-Lys-Phe-Asp-Lys-Ser-Lys-Leu-Lys-Lys-Thr-Glu-Thr-Gln-Glu-Lys-Asn-Pro-Leu-Pro-Ser-Lys-Glu-Thr-Ile-Glu-Gln-Glu-Lys-Gln-Ala-Gly-Glu-Ser |
| Molecular Weight | 4963.5 g/mol (Full Sequence) |
| Purity (HPLC) | >99.0% |
| Available Mass | 10 mg or 20 mg Lyophilized Powder |
Analytical Characteristics
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G-Actin Binding Affinity: Specifically formulated to study the sequestering of globular actin (G-actin), preventing its polymerization into F-actin to observe cellular motility.
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Low Molecular Weight Utility: Designed for high-resolution data in 3D tissue models where rapid diffusion and penetration of the peptide are required.
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Angiogenic Modulation Research: Formulated to investigate the induction of matrix metalloproteinases (MMPs) and the subsequent migration of endothelial cells.
Laboratory Application Fields
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Cellular Migration Modeling: Investigating the movement of keratinocytes and fibroblasts in simulated wound-closure assays.
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Actin-Cytoskeleton Research: Analysis of the peptide’s influence on cell shape, motility, and intracellular transport.
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Vascular Signaling Studies: Evaluation of the peptide’s role in promoting the formation of tube-like structures in endothelial cell cultures.
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Cytoprotective Pathway Mapping: Studying the impact of Thymosin analogs on reducing apoptosis and inflammatory signaling in stressed myocardial or neural models.
Storage & Handling Protocols
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Thermal Management: Store lyophilized vials at -20°C for maximum molecular integrity.
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Reconstitution: Maintain at 2-8°C post-reconstitution. This sequence is stable; however, it should be protected from high-velocity agitation to prevent mechanical denaturation.
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Stability: Use sterile 0.9% NaCl or bacteriostatic laboratory water. Avoid repeated freeze-thaw cycles after the initial reconstitution.
IMPORTANT NOTICE & DISCLAIMER
FOR LABORATORY RESEARCH USE ONLY. This product is a synthesized chemical reagent intended solely for in vitro laboratory research and analytical testing. It is NOT a drug, food, or cosmetic, and it is NOT intended for human consumption, inhalation, or any form of internal or external application to humans or animals.
Any use of this product outside of a controlled laboratory setting is strictly prohibited. The purchaser assumes all responsibility for the handling, storage, and regulatory compliance of this compound. Hudson Research Lab does not provide medical advice or instructions for human application. Keep out of reach of children.
