Description
Technical Monograph: N-AD-OX | Coenzyme Redox Reagent
Product Code: HRL-NAD-500 / HRL-NAD-1000 | Total Mass: 500 mg / 1000 mg
This high-purity Nicotinamide Adenine Dinucleotide (Oxidized Form) is an essential coenzyme found in all living cells. Engineered for advanced metabolic and cellular research, this high-concentration variant is optimized for investigating the kinetics of electron transport and the modulation of NAD-dependent enzymatic pathways.
Molecular Specification & Composition
| Attribute | Technical Detail |
| Chemical Name | $beta$-Nicotinamide Adenine Dinucleotide |
| Molecular Formula | $C_{21}H_{27}N_7O_{14}P_2$ |
| Molecular Weight | 663.4 g/mol |
| Purity (HPLC) | >99.0% |
| Available Mass | 500 mg or 1000 mg Lyophilized Powder |
Analytical Characteristics
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Redox Potential Research: Specifically formulated to study the ratio and its influence on cellular homeostasis and glycolytic flux in vitro.
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Sirtuin-Ligand Affinity: Designed to investigate the activation of NAD-dependent deacetylases (SIRT1-7) and their role in epigenetic signaling.
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Structural Integrity: Vacuum-sealed to prevent hygroscopic degradation and maintain the p-bond stability of the dinucleotide structure.
Laboratory Application Fields
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Mitochondrial Bioenergetics: Investigating the role of NAD+ in the electron transport chain and oxidative phosphorylation.
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DNA Damage Signaling: Analysis of poly(ADP-ribose) polymerase (PARP) activation and its consumption of NAD+ during repair simulations.
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Metabolic Pathway Mapping: Studying the interaction between NAD+ availability and the regulation of circadian clock genes.
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Enzymatic Kinetic Studies: Evaluation of the coenzyme’s role in over 200 different dehydrogenation reactions in cellular models.
Storage & Handling Protocols
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Thermal Management: Store lyophilized powder at -20°C for maximum molecular integrity. NAD+ is highly hygroscopic.
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Reconstitution: Maintain at 2-8°C post-reconstitution. Use immediately or aliquot and freeze to avoid repeated freeze-thaw cycles, which can lead to hydrolysis of the pyrophosphate bond.
IMPORTANT NOTICE & DISCLAIMER
FOR LABORATORY RESEARCH USE ONLY. This product is a synthesized chemical reagent intended solely for in vitro laboratory research and analytical testing. It is NOT a drug, food, or cosmetic, and it is NOT intended for human consumption, inhalation, or any form of internal or external application to humans or animals.
Any use of this product outside of a controlled laboratory setting is strictly prohibited. The purchaser assumes all responsibility for the handling, storage, and regulatory compliance of this compound. Hudson Research Lab does not provide medical advice or instructions for human application. Keep out of reach of children.
